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Our aim involved the use of a systematic design of experiments that tested the effect of salt, increasing buffer volume, adding a commercial solubilizing concoction (BugBuster ), using a mild detergent such as IGEPAL CA-630 and mechanical lysis using sonication

This convenient oral format provides an alternative to intravenous glutathione administration

Depending on the patient's history, we'll add fasting insulin, a thyroid panel, and inflammatory markers like hs-CRP

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Here, we demonstrated that the GST tag is a substrate for TG2 and that crosslinking between the GST tag and other TG2 protein substrates is responsible for the formation of GST fusion protein aggregates during pulldown experiments